Part:BBa_K2984071
Phosphite oxidoreductase (ptxD)
One restricting factor for cultivating microalgae is the high risk of contamination with bacteria, fungi or zooplankton. As our Team anticipates in building a bioreactor it is essential to sterilize the media and all containers which raise the costs of production. Loera-Quezada et al. (2016) found a way to bypass this problem by integrating Pseudomonas stutzeri WM88 gene ptxD in C. reinhardtii. It encodes a phosphite oxidoreductase which is able to oxidize phosphite into phosphate using NAD+ as a cofactor (White & Metcalf, 2007). The integration of ptxD into the genome of C. reinhardtii enables the use of phosphite as a sole phosphorus source which wasn’t possible before (Loera-Quezada et al., 2015). Loera-Quezada et al. (2016) presented a new way of microalgae production without the need for sterile conditions or antibiotics to avoid contamination with biological pollutants as they cannot use phosphite as a phosphorus source. In the future, this system could also be applied to open pond systems.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 946
- 1000COMPATIBLE WITH RFC[1000]
MoClo Overhangs
This part with B3-B4 MoClo overhangs can be found here.
This part with B3-B3 MoClo overhangs can be found here.
References
- Loera‐Quezada, M. M., Leyva‐González, M. A., Velázquez‐Juárez, G., Sanchez‐Calderón, L., Do Nascimento, M., López‐Arredondo, D., & Herrera‐Estrella, L. (2016). A novel genetic engineering platform for the effective management of biological contaminants for the production of microalgae. Plant biotechnology journal, 14(10), 2066-2076.
- White, A. K., & Metcalf, W. W. (2007). Microbial metabolism of reduced phosphorus compounds. Annu. Rev. Microbiol., 61, 379-400.
- Loera-Quezada, M. M., Leyva-González, M. A., López-Arredondo, D., & Herrera-Estrella, L. (2015). Phosphite cannot be used as a phosphorus source but is non-toxic for microalgae. Plant Science, 231, 124-130.
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